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Volume 26 Issue 12
Dec.  2019
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文章导航 >  矿物冶金与材料学报（英文）  > 2019  >  26(12): 1504-1511
Run-lan Yu, Zhen-hua Liu, Zhao-jing Yu, Xue-ling Wu, Li Shen, Yuan-dong Liu, Jiao-kun Li, Wen-qing Qin, Guan-zhou Qiu, and Wei-min Zeng, Relationship among the secretion of extracellular polymeric substances, heat resistance, and bioleaching ability of Metallosphaera sedula, Int. J. Miner. Metall. Mater., 26(2019), No. 12, pp. 1504-1511. https://doi.org/10.1007/s12613-019-1851-4
Cite this article as:
Run-lan Yu, Zhen-hua Liu, Zhao-jing Yu, Xue-ling Wu, Li Shen, Yuan-dong Liu, Jiao-kun Li, Wen-qing Qin, Guan-zhou Qiu, and Wei-min Zeng, Relationship among the secretion of extracellular polymeric substances, heat resistance, and bioleaching ability of Metallosphaera sedula, Int. J. Miner. Metall. Mater., 26(2019), No. 12, pp. 1504-1511. https://doi.org/10.1007/s12613-019-1851-4
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研究论文

Relationship among the secretion of extracellular polymeric substances, heat resistance, and bioleaching ability of Metallosphaera sedula

  • School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

  • Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China

  • 通讯作者:

    Wei-min Zeng    E-mail: Zengweimin1024@126.com

  • This paper describes the investigation of the secretion of extracellular polymeric substances (EPS) by an extremely thermoacidophilic archaea, Metallosphaera sedula (M. sedula), during the bioleaching of pyrite under different temperatures and discusses the relationship among the EPS secretion, its heat resistance, and its ability to bioleach pyrite. The investigation results indicate that the amount of extracellular proteins is significantly higher than the amount of extracellular polysaccharides in the extracted EPS whether free cells or attached cells; these results are quite different from the behavior of mesophilic Acidithiobacillus ferrooxidans. Although the growth of M. sedula is inhibited at 80℃, the bioleaching ability of M. sedula is only slightly lower than that at the optimum growth temperature of 72℃ because of the heat resistance mechanism based on EPS secretion. The secretion of more extracellular proteins is an important heat resistance mechanism of M. sedula.
    • Research Article

    Relationship among the secretion of extracellular polymeric substances, heat resistance, and bioleaching ability of Metallosphaera sedula

    + Author Affiliations
      Abstract
    • This paper describes the investigation of the secretion of extracellular polymeric substances (EPS) by an extremely thermoacidophilic archaea, Metallosphaera sedula (M. sedula), during the bioleaching of pyrite under different temperatures and discusses the relationship among the EPS secretion, its heat resistance, and its ability to bioleach pyrite. The investigation results indicate that the amount of extracellular proteins is significantly higher than the amount of extracellular polysaccharides in the extracted EPS whether free cells or attached cells; these results are quite different from the behavior of mesophilic Acidithiobacillus ferrooxidans. Although the growth of M. sedula is inhibited at 80℃, the bioleaching ability of M. sedula is only slightly lower than that at the optimum growth temperature of 72℃ because of the heat resistance mechanism based on EPS secretion. The secretion of more extracellular proteins is an important heat resistance mechanism of M. sedula.
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    • [1]
      J. Vilcáez, K. Suto, and C. Inoue, Bioleaching of chalcopyrite with thermophiles:Temperature-pH-ORP dependence, Int. J. Miner. Process., 88(2008), No. 1-2, p. 37.
      [2]
      N. Kurosawa, Y.H. Itoh, and T. Itoh, Reclassification of Sulfolobus hakonensis Takayanagi et al. 1996 as Metallosphaera hakonensis comb. nov. based on phylogenetic evidence and DNA G+C content, Int. J. Syst. Evol. Microbiol., 53(2003), No. 5, p. 1607.
      [3]
      N. Sabath, E. Ferrada, A. Barve, and A. Wagner, Growth temperature and genome size in bacteria are negatively correlated, suggesting genomic streamlining during thermal adaptation, Genome Biol. Evol., 5(2013), No. 5, p. 966.
      [4]
      D.A. Hickey and G.A.C. Singer, Genomic and proteomic adaptations to growth at high temperature, Genome Biol., 5(2004), No. 10, p. 117.
      [5]
      J. Valdes, F. Ossandon, R. Quatrini, M. Dopson, and D.S. Holmes, Draft genome sequence of the extremely acidophilic biomining bacterium Acidithiobacillus thiooxidans ATCC 19377 provides insights into the evolution of the Acidithiobacillus genus, J. Bacteriol., 193(2011), No. 24, p. 7003.
      [6]
      M.S. Urbieta, E.R. Donati, K.G. Chan, S. Shahar, L.L. Sin, and K.M. Goh, Thermophiles in the genomic era:biodiversity, science, and applications, Biotechnol. Adv., 33(2015), No. 6, p. 633.
      [7]
      G.A.C. Singer and D.A. Hickey, Thermophilic prokaryotes have characteristic patterns of codon usage, amino acid composition and nucleotide content, Gene, 317(2003), p. 39.
      [8]
      D.J. Lynn, G.A.C. Singer, and D.A. Hickey, Synonymous codon usage is subject to selection in thermophilic bacteria, Nucleic Acids Res., 30(2002), No. 19, p. 4272.
      [9]
      R.M. Daniel and D.A. Cowan, Biomolecular stability and life at high temperatures, Cell. Mol. Life Sci., 57(2000), No. 2, p. 250.
      [10]
      K. Wolfstein and L.J. Stal, Production of extracellular polymeric substances (EPS) by benthic diatoms:effect of irradiance and temperature, Mar. Ecol. Prog. Ser., 236(2002), p. 13.
      [11]
      B.A. Mckew, J.D. Taylor, T.J. McGenity, and G.J.C. Underwood, Resistance and resilience of benthic biofilm communities from a temperate salt marsh to desiccation and rewetting, ISME J., 5(2011), No. 1, p. 30.
      [12]
      D.C.O. Thornton, Effect of low pH on carbohydrate production by a marine planktonic diatom (Chaetoceros muelleri), Res. Lett. Ecol., 2009, art. No. 105901.
      [13]
      N. Mezhoud, F. Zili, N. Bouzidi, F. Helaoui, J. Ammar, and H.B. Ouada, The effects of temperature and light intensity on growth, reproduction and EPS synthesis of a thermophilic strain related to the genus Graesiella, Bioprocess. Biosyst. Eng., 37(2014), No. 11, p. 2271.
      [14]
      D. Mikkelsen, U. Kappler, R.I. Webb, R. Rasch, A.G. McEwan, and L.I. Sly, Visualisation of pyrite leaching by selected thermophilic archaea:Nature of microorganism-ore interactions during bioleaching, Hydrometallurgy, 88(2007), No. 1-4, p. 143.
      [15]
      Y. Yang, W.H. Liu, S.K. Bhargava, W.M. Zeng, and M. Chen, A XANES and XRD study of chalcopyrite bioleaching with pyrite, Miner. Eng., 89(2016), p. 157.
      [16]
      W.M. Zeng, G.Z. Qiu, H.B. Zhou, X.D. Liu, M. Chen, W.L. Chao, C.G. Zhang, and J.H. Peng, Characterization of extracellular polymeric substances extracted during the bioleaching of chalcopyrite concentrate, Hydrometallurgy, 100(2010), No. 3-4, p. 177.
      [17]
      J.I. Houghton and T. Stephenson, Effect of influent organic content on digested sludge extracellular polymer content and dewaterability, Water Res., 36(2002), No. 14, p. 3620.
      [18]
      S. Wuertz, R. Spaeth, A. Hinderberger, T. Griebe, H.C. Flemming, and P.A. Wilderer, A new method for extraction of extracellular polymeric substances from biofilms and activated sludge suitable for direct quantification of sorbed metals, Water Sci. Technol., 43(2001), No. 6, p. 25.
      [19]
      I. Bourven, E. Joussein, and G. Guibaud, Characterisation of the mineral fraction in extracellular polymeric substances (EPS) from activated sludges extracted by eight different methods, Bioresour. Technol., 102(2011), No. 14, p. 7124.
      [20]
      H. Liu and H.H.P. Fang, Extraction of extracellular polymeric substances (EPS) of sludges, J. Biotechnol., 95(2002), No. 3, p. 249.
      [21]
      L. Domínguez M. Rodríguez, and D. Prats, Effect of different extraction methods on bound EPS from MBR sludges:Part II:Influence of extraction methods over molecular weight distribution, Desalination, 262(2010), No. 1-3, p. 106.
      [22]
      B.Q. Liao, D.G. Allen, I.G. Droppo, G.G. Leppard, and S.N. Liss, Surface properties of sludge and their role in bioflocculation and settleability, Water Res., 35(2001), No. 2, p. 339.
      [23]
      R.L. Yu, C.W. Hou, A.J. Liu, T.J. Peng, M.C. Xi, W.L. Wu, L. Shen, Y.D. Liu, J.K. Li, F. Yang, G.Z. Qiu, M. Chen, and W.M. Zeng, Extracellular DNA enhances the adsorption of Sulfobacillus thermosulfidooxidans strain ST on chalcopyrite surface, Hydrometallurgy, 176(2018), p. 97.
      [24]
      W.J. Zhang, B.D. Cao, D.S. Wang, T. Ma, and D.H. Yu, Variations in distribution and composition of extracellular polymeric substances (EPS) of biological sludge under potassium ferrate conditioning:Effects of pH and ferrate dosage, Biochem. Eng. J., 106(2016), p. 37.
      [25]
      Y. Fu, H.Y. Yang, Y.J. Fang, and C.Z. Zhang, Determination of polysaccharide content in extracellular polymers of leaching bacteria, J. Cent. South Univ. Sci. Technol., 41(2010), No. 5, p. 1686.
      [26]
      J.F. Wu and C.W. Xi, Evaluation of different methods for extracting extracellular DNA from the biofilm matrix, Appl. Environ. Microbiol., 75(2009), No. 16, p. 5390.
      [27]
      R.L. Yu, A.J. Liu, Y.N. Liu, Z.J. Yu, T.J. Peng, W.L. Wu, L. Shen, Y.D. Liu, J.K. Li, X.D. Liu, G.Z. Qiu, M. Chen and W.M. Zeng, Evolution of sulfobacillus thermosulfidooxidans secreting alginate during bioleaching of chalcopyrite concentrate, J. Appl. Microbiol., 112(2017), No. 6, p. 1586.
      [28]
      C. Hoffmann, J. Leroy-Dudal, S. Patel, O. Gallet, and E. Pauthe, Fluorescein isothiocyanate-labeled human plasma fibronectin in extracellular matrix remodeling, Anal. Biochem., 372(2008), No. 1, p. 62.
      [29]
      Z.G. He, Y.P. Yang, S. Zhou, Y.H. Hu, and H. Zhong, Effect of pyrite, elemental sulfur and ferrous ions on EPS production by metal sulfide bioleaching microbes, Trans. Nonferrous Met. Soc. China, 24(2014), No. 4, p. 1171.
      [30]
      R.L. Yu, J. Liu, J.X. Tan, W.M. Zeng, L.J. Shi, G.H. Gu, W.Q. Qin, and G.Z. Qiu, Effect of pH values on the extracellular polysaccharide secreted by Acidithiobacillus ferrooxidans during chalcopyrite bioleaching, Int. J. Miner. Metall. Mater., 21(2014), No. 4, p. 311.
      [31]
      Z.J. Yu, R.L. Yu, A.J. Liu, J. Liu, W.M. Zeng, X.D. Liu, and G.Z. Qiu, Effect of pH values on extracellular protein and polysaccharide secretions of Acidithiobacillus ferrooxidans during chalcopyrite bioleaching, Trans. Nonferrous Met. Soc. China, 27(2017), No. 2, p. 406.
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