Sr2+ 对3D凝胶打印 Sr3-xMgx(PO4)2骨组织工程用复合支架的影响

刘鸿媛; 吴佳蕾; 王斯琪; 段晶; 邵慧萍

doi:10.1007/s12613-023-2638-1

Volume 30 Issue 11

Nov. 2023

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文章导航 > 矿物冶金与材料学报（英文） > 2023 > 30(11): 2236-2244

Hongyuan Liu, Jialei Wu, Siqi Wang, Jing Duan, and Huiping Shao, Effect of Sr²⁺ on 3D gel-printed Sr_3−xMg_x(PO₄)₂ composite scaffolds for bone tissue engineering, Int. J. Miner. Metall. Mater., 30(2023), No. 11, pp. 2236-2244. https://doi.org/10.1007/s12613-023-2638-1

Cite this article as:

Hongyuan Liu, Jialei Wu, Siqi Wang, Jing Duan, and Huiping Shao, Effect of Sr2+ on 3D gel-printed Sr3−xMgx(PO4)2 composite scaffolds for bone tissue engineering, Int. J. Miner. Metall. Mater., 30(2023), No. 11, pp. 2236-2244. https://doi.org/10.1007/s12613-023-2638-1

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研究论文

Sr²⁺ 对3D凝胶打印 Sr_3-xMg_x(PO₄)₂骨组织工程用复合支架的影响

北京科技大学新材料技术研究院, 北京 100083, 中国

通讯作者:
邵慧萍 E-mail: shaohp@ustb.edu.cn

文章亮点

(1)通过3D凝胶打印技术制备了多孔磷酸锶镁(Sr_3−xMg_x(PO₄)₂) (x = 2, 2.5, 3)复合支架。

(2)与纯Mg₃(PO₄)₂支架相比，掺杂了锶的支架具有更优异的综合性能。

(3)小鼠胚胎成骨前体细胞(MC3T3-E1细胞)实验显示细胞粘附在支架表面并显著增殖。

中文摘要

磷酸镁(Mg₃(PO₄)₂)是一种被广泛用于骨水泥的修复材料，具有良好的生物相容性和降解性，其降解释放的镁离子可促进骨细胞的生长和骨髓基质细胞的矿化。掺杂微量元素是提高磷酸镁生物活性的有效方法，锶(Sr)是一种存在于骨骼中的微量元素，锶离子的持续释放不仅能诱导成骨细胞分化以促进骨形成，还能抑制破骨细胞活性以减少骨吸收。此外，锶的掺入还可以改善磷酸镁的机械性能。因此通过在磷酸镁中掺杂锶离子并调整其含量，可有效提升骨组织工程支架的综合性能。本研究采用3D凝胶打印(3DGP)的方法成功制备了多孔磷酸锶镁(Sr_3−xMg_x(PO₄)₂) (x = 2, 2.5, 3) 复合支架，该工艺结合了直接喷墨打印技术和凝胶工艺，可以根据患者的需要个性化定制产品，同时具有成本低、原材料应用范围广等优点。研究结果表明，Sr_0.5Mg_2.5(PO₄)₂支架具有良好的抗压强度和体外降解率，烧结得到的Sr_0.5Mg_2.5(PO₄)₂支架孔隙率为(54.69 ± 2.64)%，抗压强度为6.34 MPa。在模拟体液中浸泡6周后，Sr_1.0Mg_2.0(PO₄)₂支架的失重率为6.96%，pH值在7.5至8.61之间变化，在人体可接受范围内。初步生物实验表明，MC3T3-E1细胞在Sr_1.0Mg_2.0(PO₄)₂支架表面具有良好的粘附性和增殖性。与纯Mg₃(PO₄)₂支架相比，掺杂了锶的支架具有更为优异的综合性能，说明Sr_1.0Mg_2.0(PO₄)₂复合支架可用于骨组织工程。

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Research Article

Effect of Sr²⁺ on 3D gel-printed Sr_3−xMg_x(PO₄)₂ composite scaffolds for bone tissue engineering

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Abstract

Abstract

Porous magnesium strontium phosphate (Sr_3−xMg_x(PO₄)₂) (x = 2, 2.5, 3) composite scaffolds were successfully prepared by three dimension gel-printing (3DGP) method in this study. The results show that Sr_0.5Mg_2.5(PO₄)₂ scaffolds had good compressive strength, and Sr_1.0Mg_2.0(PO₄)₂ scaffolds had good degradation rate in vitro. The weight loss rate of Sr_1.0Mg_2.0(PO₄)₂ scaffolds soaked in simulated body fluid (SBF) or 6 weeks was 6.96%, and pH value varied between 7.50 and 8.61, which was within the acceptable range of human body. Preliminary biological experiment shows that MC3T3-E1 cells had good adhesion and proliferation on the surface of Sr_3−xMg_x(PO₄)₂ scaffolds. Compared with pure Mg₃(PO₄)₂ scaffolds, strontium doped scaffolds had excellent comprehensive properties, which explain that Sr_3−xMg_x(PO₄)₂ composite scaffolds can be used for bone tissue engineering.
- 3D printing;
- magnesium phosphatase;
- strontium;
- porous scaffolds;
- degradability

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