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Volume 31 Issue 8
Aug.  2024
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文章导航 >  矿物冶金与材料学报（英文）  > 2024  >  31(8): 1828-1838
Yingbo Dong, Jinyu Zan, and Hai Lin, Bioleaching of vanadium from stone coal vanadium ore by Bacillus mucilaginosus: Influencing factors and mechanism, Int. J. Miner. Metall. Mater., 31(2024), No. 8, pp. 1828-1838. https://doi.org/10.1007/s12613-024-2836-5
Cite this article as:
Yingbo Dong, Jinyu Zan, and Hai Lin, Bioleaching of vanadium from stone coal vanadium ore by Bacillus mucilaginosus: Influencing factors and mechanism, Int. J. Miner. Metall. Mater., 31(2024), No. 8, pp. 1828-1838. https://doi.org/10.1007/s12613-024-2836-5
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研究论文

胶质芽孢杆菌浸出石煤钒矿中钒的影响因素与机理

  • 1)

    北京科技大学能源与环境工程学院, 北京 100083, 中国

  • 2)

    工业典型污染物资源化处理北京市重点实验室, 北京 100083, 中国



  • 通讯作者:

    林海    E-mail: linhai@ces.ustb.edu.cn

文章亮点

  • (1) 获得了不同因素对胶质芽孢杆菌浸出石煤中钒的影响规律
  • (2) 研究发现胶质芽孢杆菌的代谢产物柠檬酸、草酸在浸钒中起主导作用
  • (3) 研究发现石煤钒矿的存在可诱导胶质芽孢杆菌体内碳酸酐酶活性的提高
  • 微生物浸矿技术具有工艺简单、绿色环保、适于处理低品位矿石等优势。本论文系统研究了胶质芽孢杆菌浸出石煤钒矿中钒的影响因素与浸钒机理。通过静态浸出试验，优化得到了胶质芽孢杆菌浸钒的适宜条件为：固液比10 g⋅L−1、每100mL培养液1mL菌种接种量、反应温度30°C、蔗糖添加量20 g⋅L−1、摇床转速180 r⋅min−1。研究发现胶质芽孢杆菌对石煤中钒的浸出包括直接作用和代谢产物的间接作用，且间接作用贡献率更大，为73.8%，菌种代谢产物柠檬酸、草酸等通过酸解和络合作用实现了钒的浸出。此外发现，石煤能够促进胶质芽孢杆菌体内碳酸酐酶的催化活性，酶活性提高了1.335–1.905 U，而酶活性的提高进一步促进了代谢物有机酸的产生，总有机酸含量增加了39.31 mg⋅L−1，使浸出体系pH值降低了2.51，营造的酸性体系更有利于促进石煤中钒的浸出。
    • Research Article

    Bioleaching of vanadium from stone coal vanadium ore by Bacillus mucilaginosus: Influencing factors and mechanism

    + Author Affiliations
      Abstract
    • Vanadium and its derivatives are used in various industries, including steel, metallurgy, pharmaceuticals, and aerospace engineering. Although China has massive reserves of stone coal resources, these resources have low grades. Therefore, the effective extraction and recovery of metallic vanadium from stone coal is an important way to realize the efficient resource utilization of stone coal vanadium ore. Herein, Bacillus mucilaginosus was selected as the leaching strain. The vanadium leaching rate reached 35.5% after 20 d of bioleaching under optimal operating conditions. The cumulative vanadium leaching rate in the contact group reached 35.5%, which was higher than that in the noncontact group (9.3%). The metabolites of B. mucilaginosus, such as oxalic, tartaric, citric, and malic acids, dominated in bioleaching, accounting for 73.8% of the vanadium leaching rate. Interestingly, during leaching, the presence of stone coal stimulated the expression of carbonic anhydrase in bacterial cells, and enzyme activity increased by 1.335–1.905 U. Enzyme activity positively promoted the production of metabolite organic acids, and total organic acid content increased by 39.31 mg·L−1, resulting in a reduction of 2.51 in the pH of the leaching system with stone coal. This effect favored the leaching of vanadium from stone coal. Atomic force microscopy illustrated that bacterial leaching exacerbated corrosion on the surface of stone coal beyond 10 nm. Our study provides a clear and promising strategy for exploring the bioleaching mechanism from the perspective of microbial enzyme activity and metabolites.
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