Effect of Sr2+ on 3D gel-printed Sr3−xMgx(PO4)2 composite scaffolds for bone tissue engineering

Hongyuan Liu; Jialei Wu; Siqi Wang; Jing Duan; Huiping Shao

doi:10.1007/s12613-023-2638-1

Volume 30 Issue 11

Nov. 2023

Turn off MathJax

Article Contents

Article Navigation > International Journal of Minerals, Metallurgy and Materials > 2023 > 30(11): 2236-2244

Hongyuan Liu, Jialei Wu, Siqi Wang, Jing Duan, and Huiping Shao, Effect of Sr²⁺ on 3D gel-printed Sr_3−xMg_x(PO₄)₂ composite scaffolds for bone tissue engineering, Int. J. Miner. Metall. Mater., 30(2023), No. 11, pp. 2236-2244. https://doi.org/10.1007/s12613-023-2638-1

Cite this article as:

Hongyuan Liu, Jialei Wu, Siqi Wang, Jing Duan, and Huiping Shao, Effect of Sr2+ on 3D gel-printed Sr3−xMgx(PO4)2 composite scaffolds for bone tissue engineering, Int. J. Miner. Metall. Mater., 30(2023), No. 11, pp. 2236-2244. https://doi.org/10.1007/s12613-023-2638-1

Citation:

PDF( 4233 KB)

Research Article

Effect of Sr²⁺ on 3D gel-printed Sr_3−xMg_x(PO₄)₂ composite scaffolds for bone tissue engineering

+ Author Affiliations

Corresponding author:
Huiping Shao E-mail: shaohp@ustb.edu.cn
Received: 17 January 2023; Revised: 17 March 2023; Accepted: 29 March 2023; Available online: 30 March 2023

Abstract

Abstract

Porous magnesium strontium phosphate (Sr_3−xMg_x(PO₄)₂) (x = 2, 2.5, 3) composite scaffolds were successfully prepared by three dimension gel-printing (3DGP) method in this study. The results show that Sr_0.5Mg_2.5(PO₄)₂ scaffolds had good compressive strength, and Sr_1.0Mg_2.0(PO₄)₂ scaffolds had good degradation rate in vitro. The weight loss rate of Sr_1.0Mg_2.0(PO₄)₂ scaffolds soaked in simulated body fluid (SBF) or 6 weeks was 6.96%, and pH value varied between 7.50 and 8.61, which was within the acceptable range of human body. Preliminary biological experiment shows that MC3T3-E1 cells had good adhesion and proliferation on the surface of Sr_3−xMg_x(PO₄)₂ scaffolds. Compared with pure Mg₃(PO₄)₂ scaffolds, strontium doped scaffolds had excellent comprehensive properties, which explain that Sr_3−xMg_x(PO₄)₂ composite scaffolds can be used for bone tissue engineering.
- 3D printing;
- magnesium phosphatase;
- strontium;
- porous scaffolds;
- degradability

FullText(HTML)

Supplements(0)

References(33)

References

[1]	C. Wang, W. Huang, Y. Zhou, et al., 3D printing of bone tissue engineering scaffolds, Bioact. Mater., 5(2020), No. 1, p. 82. doi: 10.1016/j.bioactmat.2020.01.004
[2]	H.S. Ma, C. Feng, J. Chang, and C.T. Wu, 3D-printed bioceramic scaffolds: From bone tissue engineering to tumor therapy, Acta Biomater., 79(2018), p. 37. doi: 10.1016/j.actbio.2018.08.026
[3]	P.Y. Zhao, Y.Q. Liu, T.A. Li, et al., 3D printed titanium scaffolds with ordered TiO₂ nanotubular surface and mesoporous bioactive glass for bone repair, Prog. Nat. Sci., 30(2020), No. 4, p. 502. doi: 10.1016/j.pnsc.2020.08.009
[4]	R. Han, F. Buchanan, L. Ford, M. Julius, and P.J. Walsh, A comparison of the degradation behaviour of 3D printed PDLGA scaffolds incorporating bioglass or biosilica, Mater. Sci. Eng. C, 120(2021), art. No. 111755. doi: 10.1016/j.msec.2020.111755
[5]	M. Touri, F. Moztarzadeh, N.A. Abu Osman, M.M. Dehghan, and M. Mozafari, 3D-printed biphasic calcium phosphate scaffolds coated with an oxygen generating system for enhancing engineered tissue survival, Mater. Sci. Eng. C, 84(2018), p. 236. doi: 10.1016/j.msec.2017.11.037
[6]	J. Babilotte, B. Martin, V. Guduric, et al., Development and characterization of a PLGA-HA composite material to fabricate 3D-printed scaffolds for bone tissue engineering, Mater. Sci. Eng. C, 118(2021), art. No. 111334. doi: 10.1016/j.msec.2020.111334
[7]	S. Liu, L.N. Mo, G.Y. Bi, et al., DLP 3D printing porous β-tricalcium phosphate scaffold by the use of acrylate/ceramic composite slurry, Ceram. Int., 47(2021), No. 15, p. 21108. doi: 10.1016/j.ceramint.2021.04.114
[8]	A.C. Zou, H.X. Liang, C. Jiao, et al., Fabrication and properties of CaSiO₃/Sr₃(PO₄)₂ composite scaffold based on extrusion deposition, Ceram. Int., 47(2021), No. 4, p. 4783. doi: 10.1016/j.ceramint.2020.10.048
[9]	N. Kunwong, N. Tangjit, K. Rattanapinyopituk, et al., Optimization of poly (lactic-co-glycolic acid)-bioactive glass composite scaffold for bone tissue engineering using stem cells from human exfoliated deciduous teeth, Arch. Oral Biol., 123(2021), art. No. 105041. doi: 10.1016/j.archoralbio.2021.105041
[10]	L. Zhang, G.J. Yang, B.N. Johnson, and X.F. Jia, Three-dimensional (3D) printed scaffold and material selection for bone repair, Acta Biomater., 84(2019), p. 16. doi: 10.1016/j.actbio.2018.11.039
[11]	M.A. Haque and B. Chen, Research progresses on magnesium phosphate cement: A review, Constr. Build. Mater., 211(2019), p. 885. doi: 10.1016/j.conbuildmat.2019.03.304
[12]	K. Sarkar, M. Rahaman, S. Agarwal, et al., Degradability and in vivo biocompatibility of doped magnesium phosphate bioceramic scaffolds, Mater. Lett., 259(2020), art. No. 126892. doi: 10.1016/j.matlet.2019.126892
[13]	D. Pierantozzi, A. Scalzone, S. Jindal, et al., 3D printed Sr-containing composite scaffolds: Effect of structural design and material formulation towards new strategies for bone tissue engineering, Compos. Sci. Technol., 191(2020), art. No. 108069. doi: 10.1016/j.compscitech.2020.108069
[14]	N.Y. Zhong and L.P. Wang, Research progress in the osteogenetic mechanism of strontium, West China J. Stomatology, 38(2020), No. 6, p. 697. doi: 10.7518/hxkq.2020.06.016
[15]	S. Meininger, S. Mandal, A. Kumar, J. Groll, B. Basu, and U. Gbureck, Strength reliability and in vitro degradation of three-dimensional powder printed strontium-substituted magnesium phosphate scaffolds, Acta Biomater., 31(2016), p. 401. doi: 10.1016/j.actbio.2015.11.050
[16]	F.P. He, T.L. Lu, X.B. Fang, et al., Study on Mg_xSr_3−x(PO₄)₂ bioceramics as potential bone grafts, Colloids Surf. B, 175(2019), p. 158. doi: 10.1016/j.colsurfb.2018.11.085
[17]	N. Golafshan, E. Vorndran, S. Zaharievski, et al., Tough magnesium phosphate-based 3D-printed implants induce bone regeneration in an equine defect model, Biomaterials, 261(2020), art. No. 120302. doi: 10.1016/j.biomaterials.2020.120302
[18]	S. Meininger, C. Moseke, K. Spatz, et al., Effect of strontium substitution on the material properties and osteogenic potential of 3D powder printed magnesium phosphate scaffolds, Mater. Sci. Eng. C, 98(2019), p. 1145. doi: 10.1016/j.msec.2019.01.053
[19]	S.Y. Li, X.M. Pu, X.C. Chen, X.M. Liao, Z.B. Huang, and G.F. Yin, A novel bi-phase Sr-doped magnesium phosphate/calcium silicate composite scaffold and its osteogenesis promoting effect, Ceram. Int., 44(2018), No. 14, p. 16237. doi: 10.1016/j.ceramint.2018.06.009
[20]	K. Sarkar, V. Kumar, K.B. Devi, D. Ghosh, S.K. Nandi, and M. Roy, Effects of Sr doping on biodegradation and bone regeneration of magnesium phosphate bioceramics, Materialia, 5(2019), art. No. 100211. doi: 10.1016/j.mtla.2019.100211
[21]	F.P. He, T.L. Lu, X.B. Fang, et al., Effects of strontium amount on the mechanical strength and cell-biological performance of magnesium-strontium phosphate bioceramics for bone regeneration, Mater. Sci. Eng. C, 112(2020), art. No. 110892. doi: 10.1016/j.msec.2020.110892
[22]	N. Abbasi, S. Hamlet, R.M. Love, and N.T. Nguyen, Porous scaffolds for bone regeneration, J. Sci.:Adv. Mater. Devices, 5(2020), No. 1, p. 1.
[23]	M.V. Varma, B. Kandasubramanian, and S.M. Ibrahim, 3D printed scaffolds for biomedical applications, Mater. Chem. Phys., 255(2020), art. No. 123642. doi: 10.1016/j.matchemphys.2020.123642
[24]	H. Jodati, B. Yılmaz, and Z. Evis, A review of bioceramic porous scaffolds for hard tissue applications: Effects of structural features, Ceram. Int., 46(2020), No. 10, p. 15725. doi: 10.1016/j.ceramint.2020.03.192
[25]	X.Y. Ren, H.P. Shao, T. Lin, and H. Zheng, 3D gel-printing—An additive manufacturing method for producing complex shape parts, Mater. Des., 101(2016), p. 80. doi: 10.1016/j.matdes.2016.03.152
[26]	H.P. Shao, J.Z. He, T. Lin, Z.N. Zhang, Y.M. Zhang, and S.W. Liu, 3D gel-printing of hydroxyapatite scaffold for bone tissue engineering, Ceram. Int., 45(2019), No. 1, p. 1163. doi: 10.1016/j.ceramint.2018.09.300
[27]	Z.N. Zhang, H.P. Shao, T. Lin, Y.M. Zhang, J.Z. He, and L.H. Wang, 3D gel printing of porous calcium silicate scaffold for bone tissue engineering, J. Mater. Sci., 54(2019), No. 14, p. 10430. doi: 10.1007/s10853-019-03626-1
[28]	Y.M. Zhang, H.P. Shao, T. Lin, et al., Effect of Ca/P ratios on porous calcium phosphate salt bioceramic scaffolds for bone engineering by 3D gel-printing method, Ceram. Int., 45(2019), No. 16, p. 20493. doi: 10.1016/j.ceramint.2019.07.028
[29]	C.G. Chen, J.L. Wu, S.Q. Wang, and H.P. Shao, Effect of Fe₃O₄ concentration on 3D gel-printed Fe₃O₄/CaSiO₃ composite scaffolds for bone engineering, Ceram. Int., 47(2021), No. 15, p. 21038. doi: 10.1016/j.ceramint.2021.04.105
[30]	Z.N. Zhang, T. Lin, H.P. Shao, et al., Effect of different dopants on porous calcium silicate composite bone scaffolds by 3D gel-printing, Ceram. Int., 46(2020), No. 1, p. 325. doi: 10.1016/j.ceramint.2019.08.266
[31]	Y.H. Shen, W. Liu, C.Y. Wen, et al., Bone regeneration: Importance of local pH–strontium-doped borosilicate scaffold, J. Mater. Chem., 22(2012), No. 17, p. 8662. doi: 10.1039/c2jm16141a
[32]	H.P. Shao, Y.M. Zhang, T. Lin, et al., Effect of Mg²⁺ on porous Mg_xCa_3−x(PO₄)₂ composite scaffolds for bone engineering by 3D gel-printing, J. Mater. Sci., 55(2020), No. 18, p. 7870. doi: 10.1007/s10853-020-04590-x
[33]	R. Moonesi Rad, D. Atila, Z. Evis, D. Keskin, and A. Tezcaner, Development of a novel functionally graded membrane containing boron-modified bioactive glass nanoparticles for guided bone regeneration, J. Tissue Eng. Regen. Med., 13(2019), No. 8, p. 1331. doi: 10.1002/term.2877