胶质芽孢杆菌对含钒石煤的生物浸出及其吸附行为

董颖博; 崇诗佳; 林海

doi:10.1007/s12613-021-2344-9

Volume 30 Issue 2

Feb. 2023

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文章导航 > 矿物冶金与材料学报（英文） > 2023 > 30(2): 283-292

Yingbo Dong, Shijia Chong, and Hai Lin, Bioleaching and biosorption behavior of vanadium-bearing stone coal by Bacillus mucilaginosus, Int. J. Miner. Metall. Mater., 30(2023), No. 2, pp. 283-292. https://doi.org/10.1007/s12613-021-2344-9

Cite this article as:

Yingbo Dong, Shijia Chong, and Hai Lin, Bioleaching and biosorption behavior of vanadium-bearing stone coal by Bacillus mucilaginosus, Int. J. Miner. Metall. Mater., 30(2023), No. 2, pp. 283-292. https://doi.org/10.1007/s12613-021-2344-9

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研究论文

胶质芽孢杆菌对含钒石煤的生物浸出及其吸附行为

1)
北京科技大学能源与环境工程学院, 北京 100083, 中国
2)
工业典型污染物资源化处理北京市重点实验室, 北京 100083, 中国

通讯作者:
林海 E-mail: linhai@ces.ustb.edu.cn

文章亮点

(1) 获得了不同作用模式下胶质芽孢杆菌对石煤中钒的浸出规律。

(2) 揭示了不同影响因素下胶质芽孢杆菌在石煤颗粒表面的吸附特征。

(3) 系统地分析了菌体在石煤颗粒表面的吸附动力学行为与吸附机理。

中文摘要

微生物浸出含钒石煤中金属钒具有绿色、经济等优势。本文以典型硅酸盐细菌—胶质芽孢杆菌作为浸钒菌种，采用浸出和吸附试验，系统研究了胶质芽孢杆菌对含钒石煤的浸出行为及其在矿物表面的吸附特性和相关机理。研究发现，胶质芽孢杆菌在30°C条件下浸出含钒石煤28 天后，金属钒的累积浸出率达到了60.2%，表现出较好的浸钒效果。在众多影响浸出效果的因素中，含钒石煤表面的生物吸附行为至关重要，当浸出体系的pH值为5.0时，菌体与含钒石煤之间的强静电引力可有效促进生物吸附作用，处于对数生长期的胶质芽孢杆菌更具有优良的生物吸附特性，当初始菌体浓度为3.5 × 10⁸ CFU/mL时，吸附量达到3.6 × 10⁷ CFU/g，吸附率为38.9%。机理研究发现，胶质芽孢杆菌在含钒石煤表面的生物吸附过程符合Freundlich等温吸附模型和准二级吸附动力学模型，该过程属于非均质的多层吸附，且主要受化学反应控制。菌体表面的–CH₂、–CH₃、–NH₂等官能团参与了吸附过程，生物吸附作用改变了含钒石煤的表面性质，导致其等电点（IEP）向胶质芽孢杆菌的等电点靠近。

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Research Article

Bioleaching and biosorption behavior of vanadium-bearing stone coal by Bacillus mucilaginosus

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Abstract

Abstract

The recovery of vanadium (V) from stone coal by bioleaching is a promising method. The bioleaching experiments and the biosorption experiments were carried out, aiming to explore the adsorption characteristics of Bacillus mucilaginosus (B. mucilaginosus) on the surface of vanadium-bearing stone coal, and the related mechanisms have been investigated. After bioleaching at 30°C for 28 d, the cumulative leaching rate of V reached 60.2%. The biosorption of B. mucilaginosus on stone coal was affected by many factors. When the pH value of leaching system is 5.0, strong electrostatic attraction between bacteria and stone coal promoted biosorption. Bacteria in the logarithmic growth phase had mature and excellent biosorption properties. The initial bacterial concentration of 3.5 × 10⁸ CFU/mL was conducive to adhesion, with 38.9% adsorption rate and 3.6 × 10⁷ CFU/g adsorption quantity. The adsorption of B. mucilaginosus on the stone coal conformed to the Freundlich model and the pseudo-second-order kinetic model. Bacterial surface carried functional groups (–CH₂, –CH₃, –NH₂, etc.), which were highly correlated with the adsorption behavior. In addition, biosorption changed the surface properties of stone coal, resulting in the isoelectric point (IEP) approaching the bacteria. The results could provide an effective reference for the adsorption laws of bacteria on minerals.
- Bacillus mucilaginosus;
- vanadium-bearing stone coal;
- bioleaching;
- biosorption

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