Cite this article as:

Hongyuan Liu, Jialei Wu, Siqi Wang, Jing Duan, and Huiping Shao, Effect of Sr2+ on 3D gel-printed Sr3−xMgx(PO4)2 composite scaffolds for bone tissue engineering, Int. J. Miner. Metall. Mater., 30(2023), No. 11, pp.2236-2244. https://dx.doi.org/10.1007/s12613-023-2638-1
Hongyuan Liu, Jialei Wu, Siqi Wang, Jing Duan, and Huiping Shao, Effect of Sr2+ on 3D gel-printed Sr3−xMgx(PO4)2 composite scaffolds for bone tissue engineering, Int. J. Miner. Metall. Mater., 30(2023), No. 11, pp.2236-2244. https://dx.doi.org/10.1007/s12613-023-2638-1
引用本文 PDF XML SpringerLink

Sr2+ 对3D凝胶打印 Sr3-xMgx(PO4)2骨组织工程用复合支架的影响

摘要: 磷酸镁(Mg3(PO4)2)是一种被广泛用于骨水泥的修复材料,具有良好的生物相容性和降解性,其降解释放的镁离子可促进骨细胞的生长和骨髓基质细胞的矿化。掺杂微量元素是提高磷酸镁生物活性的有效方法,锶(Sr)是一种存在于骨骼中的微量元素,锶离子的持续释放不仅能诱导成骨细胞分化以促进骨形成,还能抑制破骨细胞活性以减少骨吸收。此外,锶的掺入还可以改善磷酸镁的机械性能。因此通过在磷酸镁中掺杂锶离子并调整其含量,可有效提升骨组织工程支架的综合性能。本研究采用3D凝胶打印(3DGP)的方法成功制备了多孔磷酸锶镁(Sr3−xMgx(PO4)2) (x = 2, 2.5, 3) 复合支架,该工艺结合了直接喷墨打印技术和凝胶工艺,可以根据患者的需要个性化定制产品,同时具有成本低、原材料应用范围广等优点。研究结果表明,Sr0.5Mg2.5(PO4)2支架具有良好的抗压强度和体外降解率,烧结得到的Sr0.5Mg2.5(PO4)2支架孔隙率为(54.69 ± 2.64)%,抗压强度为6.34 MPa。在模拟体液中浸泡6周后,Sr1.0Mg2.0(PO4)2支架的失重率为6.96%,pH值在7.5至8.61之间变化,在人体可接受范围内。初步生物实验表明,MC3T3-E1细胞在Sr1.0Mg2.0(PO4)2支架表面具有良好的粘附性和增殖性。与纯Mg3(PO4)2支架相比,掺杂了锶的支架具有更为优异的综合性能,说明Sr1.0Mg2.0(PO4)2复合支架可用于骨组织工程。

 

Effect of Sr2+ on 3D gel-printed Sr3−xMgx(PO4)2 composite scaffolds for bone tissue engineering

Abstract: Porous magnesium strontium phosphate (Sr3−xMgx(PO4)2) (x = 2, 2.5, 3) composite scaffolds were successfully prepared by three dimension gel-printing (3DGP) method in this study. The results show that Sr0.5Mg2.5(PO4)2 scaffolds had good compressive strength, and Sr1.0Mg2.0(PO4)2 scaffolds had good degradation rate in vitro. The weight loss rate of Sr1.0Mg2.0(PO4)2 scaffolds soaked in simulated body fluid (SBF) or 6 weeks was 6.96%, and pH value varied between 7.50 and 8.61, which was within the acceptable range of human body. Preliminary biological experiment shows that MC3T3-E1 cells had good adhesion and proliferation on the surface of Sr3−xMgx(PO4)2 scaffolds. Compared with pure Mg3(PO4)2 scaffolds, strontium doped scaffolds had excellent comprehensive properties, which explain that Sr3−xMgx(PO4)2 composite scaffolds can be used for bone tissue engineering.

 

/

返回文章
返回