The chemical composition in a cross section of a high grade pipeline slab was measured point by point (in a scale of 1 μm) using original position statistic distribution analysis (OPA). The result indicated that negative segregation strips of Si, Mn, Mo, Ni, Cr, Nb, Cu, Ti, and V exist in the two sides 24 mm away from the central line, with a width of 8-12 mm, Negative segregation inside the central line is more severe than that outside the central line, and the highest positive segregation of the elements appears closely by the inner sides of the negative segregation strips. No obvious negative segregation strip of S and P is found. Segregation of the elements in the central area is higher than that in the outer and inner arc areas. The segregation of C, Ti, S, and P is high and that of Cr, Cu, Si, and Mn is low in the slab.