Abstract: An experimental study of bioheat transfer characteristics induced by pulsed-laser irradiation was presented. The heat transfer characteristics of bio-materials, and the influences of pulse duration, power density, species of bio-materials, thickness and initial moisture content of bio-materials on heat transfer were studied in details. The experimental results indicate that the penetration and absorption of laser in bio-materials are considerable, the heat transfer inside the bio-materials should include the effects of volumetric absorption, pulse duration, power density, bio-materials thickness, and material species have a significant influence on the temperature variation.