The effect of surface microstructure of arsenopyrite on the attaching behavior of Sulfobacillus thermosulfidooxidans in the presence of additional dissolved As(III)
26 August 2020
Revised: 4 November 2020
Accepted: 27 November 2020
Available online: 28 November 2020
Abstract: The understanding of the bacterial adsorption and the evolution of biofilms on different surface structures of arsenopyrite is of great significance to clarify the mechanism of microbe-mineral interfacial interaction and the production of acidic mine drainage in the environment. In this study, the attachment of Sulfobacillus thermosulfidooxidans cells and biofilm formation on arsenopyrite with different surface structures in the presence of additional dissolved As(Ⅲ) were studied. The arsenopyrite slices with specific surface were obtained by electrochemical corrosion at 0.26 V. The scanning electronic microscopy-energy dispersion spectra (SEM-EDS) analyses indicated that the surface of arsenopyrite deficient in sulfur and iron obtained by electrochemical treatment wasn’t favorable for the initial adsorption of bacteria, and the addition of As(Ⅲ) inhibited the adsorption of microbial cells. The epifluorescence microscopy (EFM) results showed that the number of cells attaching on the arsenopyrite surface increased with time, however, when As(Ⅲ) was added, biofilm formation was delayed significantly.