Wei Chen, Sheng-hua Yin, and I.M.S.K. Ilankoon, Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching, Int. J. Miner. Metall. Mater.,(2022). https://doi.org/10.1007/s12613-020-2125-x
Cite this article as:
Wei Chen, Sheng-hua Yin, and I.M.S.K. Ilankoon, Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching, Int. J. Miner. Metall. Mater.,(2022). https://doi.org/10.1007/s12613-020-2125-x
 

Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching

+ Author Affiliations
  • Corresponding author:

    Sheng-hua Yin

  • Received: 8 April 2020Revised: 19 June 2020Accepted: 21 June 2020Available online: 24 June 2020
  • Bacterial community dynamics and copper leaching with applied forced aeration were investigated during low-grade copper sulphide bioleaching to obtain better bioleaching efficiency. Results illustrated that appropriate aeration improved bacterial concentrations and leaching efficiencies. The highest bacterial concentration and Cu2+ concentration after 14-d leaching were 7.61 × 107 cells·mL−1 and 704.9 mg·L−1, respectively, at aeration duration of 4 h·d−1. The attached bacteria played a significant role during bioleaching from 1 to 7 d. However, free bacteria dominated the bioleaching processes from 8 to 14 d. This phenomenon was mainly caused by the formation of passivation layer through Fe3+ hydrolysis along with bioleaching, which inhibited the contact between the attached bacteria and ore. Meanwhile, 16S rDNA analysis verified the effect of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans on the bioleaching process. The results demonstrate the importance of free and attached bacteria in bioleaching.
  • loading
  • [1]
    C.S. Davis-Belmar, D. Cautivo, C. Demergasso, and G. Rautenbach, Bioleaching of copper secondary sulfide ore in the presence of chloride by means of inoculation with chloride-tolerant microbial culture, Hydrometallurgy, 150(2014), p. 308.
    [2]
    W. Chen, S.H. Yin, A.X. Wu, L.M. Wang, and X. Chen, Bioleaching of copper sulfides using mixed microorganisms and its community structure succession in the presence of seawater, Bioresour. Technol., 297(2020), art. No. 122453.
    [3]
    S.H. Yin, W. Chen, J.M. Liu, and Q. Song, Agglomeration experiment of secondary copper sulfide ore, Chin. J. Eng., 41(2019), No. 9, p. 1127.
    [4]
    I.M.S.K. Ilankoon and S.J. Neethling, Liquid spread mechanisms in packed beds and heaps. The separation of length and time scales due to particle porosity, Miner. Eng., 86(2016), p. 130.
    [5]
    H.M. Lizama, S.E. Jensen, and A.W. Stradling, Dynamic microbial populations in heap leaching of zinc sulphide ore, Miner. Eng., 25(2012), No. 1, p. 54.
    [6]
    M.A. Fagan, I.E. Ngoma, R.A. Chiume, S. Minnaar, A.J. Sederman, M.L. Johns, and S.T.L. Harrison, MRI and gravimetric studies of hydrology in drip irrigated heaps and its effect on the propagation of bioleaching micro-organisms, Hydrometallurgy, 150(2014), p. 210.
    [7]
    M. Latorre, M.P. Cortés, D. Travisany, A. Di Genova, M. Budinich, A. Reyes-Jara, C. Hödar, M. González, P. Parada, R.A. Bobadilla-Fazzini, V. Cambiazo, and A. Maass, The bioleaching potential of a bacterial consortium, Bioresour. Technol., 218(2016), p. 659.
    [8]
    W. Chen, S.H. Yin, Y. Qi, X. Chen, and L.M. Wang, Effect of additives on bioleaching of copper sulfide ores, J. Cent. South Univ. Sci. Technol., 50(2019), No. 7, p. 1507.
    [9]
    C. Richter, H. Kalka, E. Myers, J. Nicolai, and H. Märten, Constraints of bioleaching in in-situ recovery applications, Hydrometallurgy, 178(2018), p. 209.
    [10]
    C.L. Brierley, Biohydrometallurgical prospects, Hydrometallurgy, 104(2010), No. 3-4, p. 324.
    [11]
    J.A. Brierley and C.L. Brierley, Present and future commercial applications of biohydrometallurgy, Hydrometallurgy, 59(2001), No. 2-3, p. 233.
    [12]
    A. Potysz, E.D. van Hullebusch, and J. Kierczak, Perspectives regarding the use of metallurgical slags as secondary metal resources — A review of bioleaching approaches, J. Environ. Manage., 219(2018), p. 138.
    [13]
    A. Henne, D. Craw, P. Vasconcelos, and G. Southam, Bioleaching of waste material from the Salobo mine, Brazil: Recovery of refractory copper from Cu hosted in silicate minerals, Chem. Geol., 498(2018), p. 72.
    [14]
    Y.G. Wang, X.H. Chen, and H.B. Zhou, Disentangling effects of temperature on microbial community and copper extraction in column bioleaching of low grade copper sulfide, Bioresour. Technol., 268(2018), p. 480.
    [15]
    L.X. Sun, X. Zhang, W.S. Tan, and M.L. Zhu, Effect of agitation intensity on the biooxidation process of refractory gold ores by Acidithiobacillus ferrooxidans, Hydrometallurgy, 127-128(2012), p. 99.
    [16]
    M. Acosta, P. Galleguillos, Y. Ghorbani, P. Tapia, Y. Contador, A. Velásquez, C. Espoz, C. Pinilla, and C. Demergasso, Variation in microbial community from predominantly mesophilic to thermotolerant and moderately thermophilic species in an industrial copper heap bioleaching operation, Hydrometallurgy, 150(2014), p. 281.
    [17]
    Y. Jia, H.Y. Sun, Q.Y. Tan, H.S. Gao, X.L. Feng, and R.M. Ruan, Linking leach chemistry and microbiology of low-grade copper ore bioleaching at different temperatures, Int. J. Miner. Metall. Mater., 25(2018), No. 3, p. 271.
    [18]
    H.L. Yang, S.S. Feng, Y. Xin, and W. Wang, Community dynamics of attached and free cells and the effects of attached cells on chalcopyrite bioleaching by Acidithiobacillus sp., Bioresour. Technol., 154(2014), p. 185.
    [19]
    S.S. Feng, H.L. Yang, and W. Wang, Insights to the effects of free cells on community structure of attached cells and chalcopyrite bioleaching during different stages, Bioresour. Technol., 200(2016), p. 186.
    [20]
    H. Wang, X. Zhang, M.L. Zhu, and W.S. Tan, Effects of dissolved oxygen and carbon dioxide under oxygen-rich conditions on the biooxidation process of refractory gold concentrate and the microbial community, Miner. Eng., 80(2015), p. 37.
    [21]
    B.Q. Yu, J. Kou, Y. Xing, and C.B. Sun, Enhanced extraction of copper from cupriferous biotite by organic intercalation, Hydrometallurgy, 192(2020), art. No. 105286.
    [22]
    A. Caramento, Cultivating backward linkages to Zambia's copper mines: Debating the design of, and obstacles to, local content, Extr. Ind. Soc., 7(2020), No. 2, p. 310.
    [23]
    P. Mwaanga, M. Silondwa, G. Kasali, and P.M. Banda, Preliminary review of mine air pollution in Zambia, Heliyon, 5(2019), No. 9, art. No. e02485.
    [24]
    A.G. Guezennec, C. Joulian, J. Jacob, A. Archane, D. Ibarra, R. de Buyer, F. Bodénan, and P. d’Hugues, Influence of dissolved oxygen on the bioleaching efficiency under oxygen enriched atmosphere, Miner. Eng., 106(2017), p. 64.
    [25]
    S.H. Yin, W. Chen, X. Chen, and L.M. Wang, Bacterial-mediated recovery of copper from low-grade copper sulphide using acid-processed rice straw, Bioresour. Technol., 288(2019), art. No. 121605.
    [26]
    J.Y. Liu, X.X. Xiu, and P. Cai, Study of formation of jarosite mediated by thiobacillus ferrooxidans in 9K medium, Procedia Earth Planet. Sci., 1(2009), No. 1, p. 706.
    [27]
    S.S. Feng, H.L. Yang, X. Zhan, and W. Wang, Novel integration strategy for enhancing chalcopyrite bioleaching by Acidithiobacillus sp. in a 7-L fermenter, Bioresour. Technol., 161(2014), p. 371.
    [28]
    S.S. Feng, H.L. Yang, and W. Wang, Improved chalcopyrite bioleaching by Acidithiobacillus sp. via direct step-wise regulation of microbial community structure, Bioresour. Technol., 192(2015), p. 75.
    [29]
    S.H. Yin, L.M. Wang, A.X. Wu, X. Chen, and R.F. Yan, Research progress in enhanced bioleaching of copper sulfides under the intervention of microbial communities, Int. J. Miner. Metall. Mater., 26(2019), No. 11, p. 1337.
    [30]
    H. Osorio, S. Mangold, Y. Denis, I. Ñancucheo, M. Esparza, D.B. Johnson, V. Bonnefoy, M. Dopson, and D.S. Holmes, Anaerobic sulfur metabolism coupled to dissimilatory iron reduction in the extremophile Acidithiobacillus ferrooxidans, Appl. Environ. Microbiol., 79(2013), No. 7, p. 2172.
    [31]
    M. Dopson and D.B. Johnson, Biodiversity, metabolism and applications of acidophilic sulfur-metabolizing microorganisms, Environ. Microbiol., 14(2012), No. 10, p. 2620.
    [32]
    H.M. Lizama, Copper bioleaching behaviour in an aerated heap, Int. J. Miner. Process., 62(2001), No. 1-4, p. 257.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(4)

    Share Article

    Article Metrics

    Article views (1617) PDF downloads(20) Cited by()
    Proportional views

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return